PLAMORF: Long-distance RNA signalling in plants

Consortium

Objectives

PLAMORF is…

Challenge

PLAMORF focuses on Plant Mobile RNAs: Function, Transport and Features.

An essential consequence of multi-cellularity is the need for intercellular and tissue-wide communication, which happens through transport of signalling molecules. In higher plants long-distance transport of signalling molecules occurs mainly via  the phloem.

In addition to small molecules, a remarkably large number of macromolecules such as micro RNAs (miRNAs), protein producing messenger RNAs (mRNAs), and RNA-binding proteins (RBPs) have been identified in the phloem, representing additional classes of signalling molecules.

Research questions and approach

  • How are mobile RNAs selected for transport?
  • How specific is RNA mobility?
  • What determines their destination?
  • How are these signals processed in the destination cells?

We develop predictive models, using single cell transcriptomics to establish cell-type specific RNA transport and motifs, and study the structure, affinity, and functions of phloem transported RNAs associated proteins. We combine the advantages of the agronomically important oilseed rape with the well-established A. thaliana belonging to the same plant family.

The ERC

PLAMORF is funded by the European Research Council (ERC). The ERC, set up by the EU in 2007, is the premiere European funding organisation for excellent frontier research. Every year, it selects and funds the very best, creative researchers of any nationality and age, to run projects based in Europe.

The ERC offers four core grant schemes: Starting, Consolidator, Advanced and Synergy Grants. With its additional Proof of Concept grant scheme, the ERC helps grantees to bridge the gap between grantees’ pioneering research and early phases of its commercialisation.

News & Blog

PREPRINT: HSC70.1 chaperone binds own mRNA for feed-back regulation

PLAMORF preprint: about graft-mobile HSC70 chaperone, its homeostatic feed-back regulation by binding its own mRNA and phenotype complementation only seen with mobile HSC70 mRNA

PROTOCOL: Arabidopsis Callus Grafting Method to Test Cell-to-Cell Mobility of Proteins

Would you like to know, if the plant protein you are working on, can move from one cell into the next through plasmodesmata?

3D printable moulds for callus cultivation

3D Printable files to create moulds for Arabidopsis callus co-cultivation and for callus cryosectioning are now available from Zenodo.

Twitter feed

👉The #EC is asking for our feedback on targeted mutagenesis of #plants! 🧬🌱 #CRISPR #plantscience #haveyoursay https://t.co/9LtiSuNgT2
@plamorf
@ASPB_ECPS: With travel costs as high as they are, ECPS is handing out some additional small travel grants to Early Career folks to attend Plant Biology 2022! Apps will only be open for a couple days and the selections will be made quickly. This is going to be a great conference for ECRs! https://t.co/PICn4PpAM9
@plamorf
@GoeHensel: Happy to announce a 2nd training school of WG1 of @COST_PlantEd taking place @HHU_de directly after the congress. Together with @SadiyeHayta @MarkSmedley15, Vladislava Galovic, Thomas Jacobs and Katrijn Van Laere, we will more focus on #CRISPR design etc. https://t.co/9XVizi5osn https://t.co/slbgY9MBU8
@plamorf

PLAMORF in a nutshell

Fact box

Title: Plant mobile RNAs: function, transport and features (PLAMORF)
Start: 1.4.2019
Duration: 6 years
Keywords: plant, tranport, mobile RNA, signalling, phloem, RNA-binding proteins, oilseed rape, A. thaliana
Budget: 6.1 million
Funding: ERC synergy grant

Research groups:

Friedrich Kragler, Max-Planck-Institut für Molekulare Pflanzenphysiologie, Golm, Germany
Julia Kehr, Universität Hamburg, Hamburg, Germany
Richard Morris, John Innes Centre, Norwich, United Kingdom

 

Acknowledgements

This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (Grant agreement No. 810131)