PROTOCOL: Arabidopsis Callus Grafting Method to Test Cell-to-Cell Mobility of Proteins

Would you like to know, if the plant protein you are working on, can move from one cell into the next through plasmodesmata?

Use co-culturing of Arabidopsis calli, following the simple protocol just described by the Kragler group in the 2nd edition of the Plasmodesmata Book:

https://link.springer.com/protocol/10.1007/978-1-0716-2132-5_20

Machin F., Hasbioğlu Y., Kragler F. (2022) An Arabidopsis Callus Grafting Method to Test Cell-to-Cell Mobility of Proteins. In: Benitez-Alfonso Y., Heinlein M. (eds) Plasmodesmata. Methods in Molecular Biology, vol 2457. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2132-5_20