Another tool to find out more about cell to cell movement of macromolecules: Yağmur Hasbioğlu, Stephanie Weber, Friedrich Kragler and Frank Qasim Machin just reported in The Plant Journal about Arabidopsis callus grafts as a system to study symplasmic intercellular macromolecular transport and gene silencing spread.
Read the abstract or download the full publication!
Figure 3: Fixation and sectioning allows images to be taken of cell walls with several PD complexes as revealed by PD-specific proteins.
(a) CLSM images of cryosections of 35S:MP17-GFP callus. Green indicates MP17-GFP and blue is cell walls stained with Calcofluor. Scale bar = 0.5 μm.
(b) CLSM images of cryosection of 35S:PDLP5-Cherry callus. Red indicates PDLP5-Cherry and blue indicates cell walls stained with Calcofluor. Scale bar = 0.5 μm.
(c–f) CLSM image of a cryosection of a callus graft between 35S:MP17-GFP (c) and 35S:PDLP5-Cherry (d), stained with Calcofluor (e) and merged (f). Scale bars = 0.5 μm.
